英语

scopus和web of science上都有全文可以下的.

只有摘要，没有全文。
Formation of diacetyl by cell-free extracts of Leuconostoc lactis

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Kieran N. Jordana, , , Margie O'donoghueb, Seamus Condonb and timothy M. Cogana

a Teagasc, National Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Ireland

b Department of Microbiology, University College Cork, Cork, Ireland

Received 28 May 1996; revised 19 July 1996; accepted 14 August 1996. ; Available online 22 March 1999.

Abstract
Diacetyl formation was linear with time and with protein concentration when a cell-free extract of Leuconostoc lactis NCW1 was added to a buffer system containing pyruvate, thiamine pyrophosphate and MgS4 (final concentrations 60 mM, 0.11 mM and 0.22 mM, respectively). No diacetyl was detected in the absence of pyruvate or cell-free extract and no increase in diacetyl formation was detected on the addition of acetyl-CoA. When 2-acetolactate (1.6 mM) was the substrate, autodecarboxylation to diacetyl and acetoin occurred under aerobic and anaerobic conditions. When cell-free extract was added, decarboxylation of 2-acetolactate to acetoin and diacetyl increased 4–6-fold, under aerobic and anaerobic conditions. When the cell-free extract was boiled, diacetyl formation from 2-acetolactate was reduced to the level of autodecarboxylation. The results suggest that diacetyl is formed enzymatically in the presence and absence of oxygen, as well as spontaneously, from 2-acetolactate.